	US 12,319,926 B2
	Recombinant humanized collagen type I alpha-1 (rhCol1A1), and expression vector and use thereof
Zhidong Guo, Heze (CN); Hongwei Ma, Heze (CN); and Jiajia Shao, Heze (CN)
Assigned to SHANDONG D-NUTRIMEC BIOMEDICAL CO., LTD., Heze (CN)
Appl. No. 18/834,523
Filed by Shandong D-Nutrimec Biomedical Co., Ltd., Heze (CN)
PCT Filed Dec. 26, 2023, PCT No. PCT/CN2023/142064 § 371(c)(1), (2) Date Jul. 30, 2024, PCT Pub. No. WO2024/159985, PCT Pub. Date Aug. 8, 2024.
Claims priority of application No. 202310055281.X (CN), filed on Feb. 4, 2023.
Prior Publication US 2025/0109406 A1, Apr. 3, 2025
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/85 (2006.01); C07K 14/78 (2006.01); C12N 5/00 (2006.01); C12N 5/071 (2010.01); C12N 15/66 (2006.01)

CPC C12N 15/85 (2013.01) [C07K 14/78 (2013.01); C12N 5/0037 (2013.01); C12N 5/0686 (2013.01); C12N 15/66 (2013.01); C12N 2500/02 (2013.01); C12N 2523/00 (2013.01); C12N 2527/00 (2013.01); C12N 2800/107 (2013.01); C12N 2800/80 (2013.01)]

2 Claims

1. An expression vector for recombinant humanized collagen type I alpha-1 (rhCol1A1), wherein the expression vector for rhCol1A1 is a pcDNA3.1 expression vector comprising the DNA sequence of SEQ ID NO: 4 and the DNA sequence of SEQ ID NO: 3, wherein the rhCol1A1 has the amino acid sequence of SEQ ID NO: 1;

the DNA sequence of SEQ ID NO: 3 is inserted between BamH I and Xho I restriction sites of the pcDNA3.1 expression vector; and

the DNA sequence of SEQ ID NO: 4 is inserted between Nhe I and BamH I restriction sites of the expression vector pcDNA3.1.