US 12,319,922 B2
Methods and compositions for short stature plants through manipulation of gibberellin metabolism to increase harvestable yield
Edwards M. Allen, O'Fallon, MO (US); Jayanand Boddu, St. Louis, MO (US); Charles R. Dietrich, Chesterfield, MO (US); Alexander Goldshmidt, Davis, CA (US); Miya Howell, Ballwin, MO (US); Kevin R. Kosola, Wildwood, MO (US); Sivalinganna Manjunath, Chesterfield, MO (US); Anil Neelam, St. Louis, MO (US); Linda Rymarquis, High Ridge, MO (US); Thomas L. Slewinski, Chesterfield, MO (US); Tyamagondlu V. Venkatesh, St. Louis, MO (US); and Huai Wang, St. Louis, MO (US)
Assigned to MONSANTO TECHNOLOGY LLC, St. Louis, MO (US)
Filed by MONSANTO TECHNOLOGY LLC, St. Louis, MO (US)
Filed on Apr. 5, 2022, as Appl. No. 17/713,344.
Application 17/713,344 is a continuation of application No. 16/847,244, filed on Apr. 13, 2020, granted, now 11,319,550.
Application 16/847,244 is a continuation of application No. 15/679,699, filed on Aug. 17, 2017, granted, now 10,724,047, issued on Jul. 28, 2020.
Claims priority of provisional application 62/502,313, filed on May 5, 2017.
Claims priority of provisional application 62/442,377, filed on Jan. 4, 2017.
Claims priority of provisional application 62/376,298, filed on Aug. 17, 2016.
Prior Publication US 2022/0364108 A1, Nov. 17, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/82 (2006.01)
CPC C12N 15/8261 (2013.01) [C12N 15/8273 (2013.01); C12N 15/8297 (2013.01)] 83 Claims
 
1. A recombinant DNA construct comprising a transcribable DNA sequence encoding a non-coding RNA molecule, wherein the non-coding RNA molecule comprises a targeting sequence that is:
(a) at least 80% complementary to at least 19 consecutive nucleotides of a first mRNA molecule encoding a first endogenous gibberellin (GA) oxidase protein in a corn plant, the first endogenous GA oxidase protein being at least 90% identical to SEQ ID NO: 9; and
(b) at least 80% complementary to at least 19 consecutive nucleotides of a second mRNA molecule encoding a second endogenous GA oxidase protein in a corn plant, the second endogenous GA oxidase protein being at least 90% identical to SEQ ID NO: 15;
wherein the transcribable DNA sequence is operably linked to a leaf promoter.