US 12,319,919 B2
Synthetic ethylene-responsive binding element and uses thereof
Anna N. Stepanova, Cary, NC (US); Jose M. Alonso, Cary, NC (US); Josefina Patricia Fernandez-Moreno, Raleigh, NC (US); Elena Zemlyanskaya, Novosibirsk (RU); and Victor Levitsky, Novosibirsk (RU)
Assigned to NORTH CAROLINA STATE UNIVERSITY, Raleigh, NC (US); and INSTITUTE OF CYTOLOGY AND GENETICS, SIBERIAN BRANCH OF RUSSIAN ACADEMY OF SCIENCES, Novosibirsk (RU)
Appl. No. 18/002,134
Filed by NORTH CAROLINA STATE UNIVERSITY, Raleigh, NC (US); and INSTITUTE OF CYTOLOGY AND GENETICS, SIBERIAN BRANCH OF RUSSIAN ACADEMY OF SCIENCES, Novosibirsk (RU)
PCT Filed Jun. 30, 2021, PCT No. PCT/US2021/039766
§ 371(c)(1), (2) Date Dec. 16, 2022,
PCT Pub. No. WO2022/006203, PCT Pub. Date Jan. 6, 2022.
Claims priority of provisional application 62/705,516, filed on Jul. 1, 2020.
Prior Publication US 2023/0235346 A1, Jul. 27, 2023
Int. Cl. C12N 15/82 (2006.01); A01H 6/20 (2018.01); A01H 6/82 (2018.01)
CPC C12N 15/8238 (2013.01) [A01H 6/20 (2018.05); A01H 6/823 (2018.05); A01H 6/825 (2018.05); C12N 15/8242 (2013.01); C12N 2320/50 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A recombinant nucleic acid construct comprising two or more directly adjoining ethylene binding sequences (EBS), wherein each of the EBS is a sequence of Formula I (SEQ ID NO:48):
X—N1—N2—N3—N4—N5—N6—N7—N8—N9—N10—N11—Y  (I)
wherein:
N1 is A or G;
N2 is T;
N3 is G, T or A;
N4 is C;
N5 is A;
N6 is A or T;
N7 is T;
N8 is G;
N9 is A, T or C;
N10 is A;
N11 is T or C; and
X and Y are each independently present or absent and when present is each independently a spacer sequence comprising 1 to 50 nucleotides.