	US 12,319,917 B2
	Expression modulating elements and methods of use
Priyanka Bhyri, Johnston, IA (US); Amitabh Mohanty, Hyderabad (IN); and Phanikanth Turlapati, Hyderabad (IN)
Assigned to PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
Appl. No. 17/288,509
Filed by PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
PCT Filed Nov. 5, 2019, PCT No. PCT/US2019/059886 § 371(c)(1), (2) Date Apr. 23, 2021, PCT Pub. No. WO2020/097092, PCT Pub. Date May 14, 2020.
Claims priority of provisional application 62/756,354, filed on Nov. 6, 2018.
Prior Publication US 2021/0388369 A1, Dec. 16, 2021
Int. Cl. C12N 15/82 (2006.01); C07K 14/41 (2006.01); C07K 14/415 (2006.01)

CPC C12N 15/8216 (2013.01) [C07K 14/415 (2013.01)]

15 Claims

1. A plant cell comprising at least one copy of an expression modulating element operably linked to a polynucleotide, the expression modulating element consisting essentially of a nucleic acid sequence having at least 98% sequence identity to SEQ ID NO: 1, and the polynucleotide comprising a promoter, the promoter heterologous to the expression modulating element, and a gene of interest or a functional RNA, wherein expression of the gene of interest or functional RNA is increased as compared to a control plant cell not comprising the expression modulating element.