US 12,319,908 B1
System and method for the activation of active ion transporters without the consumption of adenosine triphosphate (ATP) molecules
Wei Chen, Tampa, FL (US)
Assigned to University of South Florida, Tampa, FL (US)
Filed by University of South Florida, Tampa, FL (US)
Filed on Mar. 17, 2022, as Appl. No. 17/697,582.
Claims priority of provisional application 63/162,171, filed on Mar. 17, 2021.
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 13/00 (2006.01); A61N 1/36 (2006.01); C12M 1/42 (2006.01)
CPC C12N 13/00 (2013.01) [C12M 35/02 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method for controlling active ion transporters, the method comprising:
applying an oscillating electric field to one or more active ion transporters, wherein the oscillating electric field comprises three serially applied phases and wherein applying the oscillating electric field comprises;
applying a synchronization phase to synchronize the active ion transporters to a physiological turnover rate of the active ion transporters down to individual steps within a running cycle with a net-consumption of adenosine triphosphate (ATP) substantially equal to zero, wherein applying the synchronization phase comprises, applying an oscillating electric field to synchronize an ion-pumping in half-cycle and an ion-extrusion half-cycle of the active ion transporters, wherein the ion-pumping in half-cycle of the active ion transporter sis in a negative half-cycle and the ion-extrusion half-cycle of the active ion transporters is in a positive half-cycle of the oscillating electric field and wherein applying the synchronization phase further comprises:
applying an activation overshoot electric pulse at a start of the negative half-cycle and at a start of the positive half-cycle of the oscillating electric field;
applying an energy-trap overshoot electric pulse at an end of the negative half-cycle and at an end of the positive half-cycle of the oscillating electric field; and
applying an electric field plateau in between the activation overshoot electric pulse and the energy-trap overshoot electric pulse, wherein the activation overshoot electric pulse, the electric field plateau and the energy-trap overshoot electric pulse result in the synchronization of the active ion transporters down to individual steps within the running cycle;
applying a modulation phase to modulate the synchronized active ion transporters to a predetermined target turnover rate; and
applying a maintenance phase to maintain the synchronized active ion transporters at the predetermined target turnover rate for a predetermined duration of time.