| CPC C12N 1/20 (2013.01) | 5 Claims |
|
1. A medium composition for culturing Faecalibacterium prausnitzii phylogroup II, to a density of 1010 to 1011 CFUs/mL comprising vegetable peptone, yeast extract, a phosphate compound, a carbonate compound, cyanocobalamin, L-cysteine, ammonium acetate, and maltose,
wherein the vegetable peptone is present in an amount of 10-20 g/L, the yeast extract is present in an amount from 5-10 g/L, the phosphate compound is selected from disodium phosphate (Na2HPO4), dipotassium phosphate (K2HPO4) or potassium phosphate (KH2PO4) or mixtures thereof, and is present in an amount to provide a medium composition pH of 6.5 to 8.0, the maltose is present in an amount from 2.5-30 g/L, the carbonate compound is potassium hydrogen carbonate and is present in an amount from 2-6 g/L, and the ammonium acetate is present in an amount from 2-16 g/L.
|