US 12,319,901 B2
Isolating live cells after high-throughput, long-term, time-lapse microscopy
Scott Luro, Cambridge, MA (US); Burak Okumus, Cambridge, MA (US); and Johan Paulsson, Cambridge, MA (US)
Assigned to PRESIDENT AND FELLOWS OF HARVARD COLLEGE, Cambridge, MA (US)
Appl. No. 17/620,636
Filed by President and Fellows of Harvard College, Cambridge, MA (US)
PCT Filed Jun. 22, 2020, PCT No. PCT/US2020/038867
§ 371(c)(1), (2) Date Dec. 17, 2021,
PCT Pub. No. WO2020/257746, PCT Pub. Date Dec. 24, 2020.
Claims priority of provisional application 62/864,091, filed on Jun. 20, 2019.
Prior Publication US 2022/0348854 A1, Nov. 3, 2022
Int. Cl. C12M 3/06 (2006.01); C12M 1/00 (2006.01)
CPC C12M 23/16 (2013.01) [C12M 47/04 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A microfluidic device for use in analyzing cells and extracting one or more cells of interest, the microfluidic device comprising:
a substrate;
a cell flow layer coupled to the substrate, the cell flow layer including:
a growth channel having a main portion and an inlet valve portion, the inlet valve portion of the growth channel being configured to aid in selectively controlling flow into the main portion of the growth channel;
a collection channel having a main portion and an outlet valve portion, the outlet valve portion of the collection channel being configured to aid in selectively controlling flow out of the main portion of the collection channel;
one or more bridge channels coupling the main portion of the growth channel with the main portion of the collection channel, each of the one or more bridge channels including a bridge valve portion configured to aid in selectively controlling flow between the growth channel and the collection channel; and
a control layer coupled to the cell flow layer and configured to aid in actuating (i) the bridge valve portion of each of the one or more bridge channels, (ii) the inlet valve portion of the growth channel, and (iii) the outlet valve portion of the collection channel.