US 12,319,740 B2
Methods for production of tissue resident memory-like T cells and use thereof
Cassian Yee, Houston, TX (US); and Farah Hasan, Houston, TX (US)
Assigned to BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM, Austin, TX (US)
Appl. No. 17/286,086
Filed by Board of Regents, The University of Texas System, Austin, TX (US)
PCT Filed Oct. 18, 2019, PCT No. PCT/US2019/057016
§ 371(c)(1), (2) Date Apr. 16, 2021,
PCT Pub. No. WO2020/081987, PCT Pub. Date Apr. 23, 2020.
Claims priority of provisional application 62/846,270, filed on May 10, 2019.
Claims priority of provisional application 62/747,523, filed on Oct. 18, 2018.
Prior Publication US 2021/0355443 A1, Nov. 18, 2021
Int. Cl. C07K 16/28 (2006.01); A61K 39/395 (2006.01); A61K 40/11 (2025.01); A61K 40/32 (2025.01); A61K 40/42 (2025.01); C12N 5/0783 (2010.01)
CPC C07K 16/2878 (2013.01) [A61K 39/3955 (2013.01); A61K 40/11 (2025.01); A61K 40/32 (2025.01); A61K 40/4272 (2025.01); A61K 40/4273 (2025.01); C12N 5/0636 (2013.01); C12N 2501/15 (2013.01); C12N 2501/2315 (2013.01); C12N 2501/73 (2013.01); C12N 2502/1121 (2013.01)] 18 Claims
 
1. An in vitro method for producing tissue resident memory-like T cells (TRM-like T cells) comprising:
(a) obtaining a starting population of T cells;
(b) culturing the starting population of T cells in hypoxic conditions or in the presence of a hypoxia-inducing agent to generate early effector cells, wherein the culturing is in media essentially free of transforming growth factor beta 1 (TGF-β1); and
(c) further culturing the early effector cells in the presence of TGF-β1 to produce TRM-like T cells.