US 12,318,486 B2
Dry powder composition comprising long-chain RNA
Fabian Johannes Eber, Stuttgart (DE); Benyamin Yazdan Panah, Tübingen (DE); Stefanie Sewing, Tübingen (DE); Thomas Ketterer, Gomaringen (DE); Thorsten Mutzke, Reutlingen (DE); Tilmann Roos, Kusterdingen (DE); Michael Sonntag, Tübingen (DE); Michael Wiggenhorn, Munich (DE); and Katharina Kolland, Augsburg (DE)
Assigned to CureVac SE, Tübingen (DE)
Filed by CureVac SE, Tübingen (DE)
Filed on Jul. 11, 2022, as Appl. No. 17/811,808.
Application 17/811,808 is a division of application No. 16/998,259, filed on Aug. 20, 2020, granted, now 11,433,027, issued on Aug. 17, 2022.
Application 16/998,259 is a division of application No. 16/679,536, filed on Nov. 11, 2019, granted, now 11,179,337, issued on Nov. 23, 2021.
Application 16/679,536 is a division of application No. 15/575,284, granted, now 10,517,827, issued on Dec. 31, 2019, previously published as PCT/EP2016/000842, filed on May 20, 2016.
Claims priority of application No. 15001517 (EP), filed on May 20, 2015; and application No. PCT/EP2015/002019 (WO), filed on Oct. 13, 2015.
Prior Publication US 2022/0387320 A1, Dec. 8, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C07H 21/04 (2006.01); A61K 9/16 (2006.01); A61K 39/145 (2006.01); A61K 48/00 (2006.01); A61P 31/00 (2006.01); A61P 31/04 (2006.01); A61P 31/12 (2006.01); A61P 33/02 (2006.01); A61P 35/00 (2006.01); A61P 37/06 (2006.01); A61P 37/08 (2006.01); A61K 39/00 (2006.01)
CPC A61K 9/16 (2013.01) [A61K 9/1682 (2013.01); A61K 39/145 (2013.01); A61K 48/005 (2013.01); A61K 48/0091 (2013.01); A61P 31/00 (2018.01); A61P 31/04 (2018.01); A61P 31/12 (2018.01); A61P 33/02 (2018.01); A61P 35/00 (2018.01); A61P 37/06 (2018.01); A61P 37/08 (2018.01); A61K 2039/53 (2013.01); A61K 48/00 (2013.01)] 16 Claims
 
1. A dry powder composition comprising a long-chain RNA molecule that is 500 to 20,000 nucleotides in length and is formulated with a cationic or polycationic compound, wherein the long-chain RNA molecule comprises at least one open reading frame (ORF) encoding a protein, wherein:
(i) said dry powder has a residual moisture content of less than 2%-5% (w/w); and
(ii) comprises a plurality of particles having a median particle size, in a volume weighted distribution of the composition, in a range from 1 μm and 20 μm.