	US 12,318,439 B2
	Attenuating viral mutations in protein genes
Jaclyn Kaiser, Galveston, TX (US); Tian Wang, Galveston, TX (US); Alan Barrett, Galveston, TX (US); and Kassandra Carpio, Galveston, TX (US)
Assigned to Board of Regents, The University of Texas System, Austin, TX (US)
Filed by BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM, Austin, TX (US)
Filed on May 5, 2022, as Appl. No. 17/737,991.
Claims priority of provisional application 63/186,160, filed on May 9, 2021.
Prior Publication US 2022/0354942 A1, Nov. 10, 2022
Int. Cl. A61K 39/12 (2006.01); A61K 39/00 (2006.01); A61P 31/14 (2006.01); C07K 14/18 (2006.01); C12N 7/00 (2006.01)

CPC A61K 39/12 (2013.01) [A61P 31/14 (2018.01); C07K 14/18 (2013.01); C12N 7/00 (2013.01); A61K 39/00 (2013.01); A61K 2039/5254 (2013.01); C12N 2770/24134 (2013.01)]

19 Claims

1. An attenuated flavivirus genome comprising a NS4B gene segment encoding a mutant NS4B protein having an amino acid substitution analogous to an alanine or glycine substitution at proline 54 of the West Nile Virus (WNV) NS4B protein, SEQ ID NO: 19.