	US 12,318,431 B2
	Cytokine conjugates for the treatment of proliferative and infectious diseases
Jerod Ptacin, San Diego, CA (US); Carolina E. Caffaro, San Diego, CA (US); and Marcos Milla, Carlsbad, CA (US)
Assigned to SYNTHORX, INC., Bridgewater, NJ (US)
Filed by Synthorx, Inc., La Jolla, CA (US)
Filed on Jun. 1, 2023, as Appl. No. 18/327,535.
Application 16/803,816 is a division of application No. 16/434,999, filed on Jun. 7, 2019, granted, now 10,610,571.
Application 18/327,535 is a continuation of application No. 16/803,816, filed on Feb. 27, 2020, granted, now 11,701,407.
Application 16/434,999 is a continuation of application No. PCT/US2018/045257, filed on Aug. 3, 2018.
Claims priority of provisional application 62/540,781, filed on Aug. 3, 2017.
Prior Publication US 2024/0082359 A1, Mar. 14, 2024
Int. Cl. A61K 38/00 (2006.01); A61K 38/20 (2006.01); A61K 47/54 (2017.01); A61K 47/60 (2017.01); A61K 47/61 (2017.01); A61K 47/64 (2017.01); A61K 47/65 (2017.01); A61K 47/68 (2017.01); C07K 14/55 (2006.01); C07K 14/715 (2006.01)

CPC A61K 38/2013 (2013.01) [A61K 47/542 (2017.08); A61K 47/60 (2017.08); A61K 47/61 (2017.08); A61K 47/643 (2017.08); A61K 47/644 (2017.08); A61K 47/65 (2017.08); A61K 47/68 (2017.08); C07K 14/55 (2013.01); C07K 14/7155 (2013.01); A61K 38/00 (2013.01)]

21 Claims

1. A polynucleic acid molecule encoding a modified interleukin 2 (IL-2) polypeptide, wherein the modified IL-2 polypeptide comprises:

an amino acid sequence that is at least 80% identical to SEQ ID NO: 1, and at least one unnatural amino acid at position K35, T37, R38, T41, F42, K43, F44, Y45, E60, E61, E62, K64, P65, E68, V69, N71, L72, M104, C105, or Y107 in reference to the amino acid positions as set forth in SEQ ID NO: 1,

wherein the polynucleic acid molecule comprises a synthetic codon comprising an unnatural nucleotide, and wherein the synthetic codon encodes the at least one unnatural amino acid.