US 12,317,907 B2
Hydrolysate of water soluble insect proteins and method for preparation thereof
Aman Paul, Rijen (NL)
Assigned to Protix B.V., Dongen (NL)
Appl. No. 17/641,821
Filed by Protix B.V., Dongen (NL)
PCT Filed Sep. 16, 2020, PCT No. PCT/NL2020/050572
§ 371(c)(1), (2) Date Mar. 10, 2022,
PCT Pub. No. WO2021/054824, PCT Pub. Date Mar. 25, 2021.
Claims priority of application No. 2023838 (NL), filed on Sep. 17, 2019; application No. 2024481 (NL), filed on Dec. 17, 2019; application No. 2025546 (NL), filed on May 11, 2020; and application No. 2025547 (NL), filed on May 11, 2020.
Prior Publication US 2024/0041068 A1, Feb. 8, 2024
Int. Cl. A61K 35/64 (2015.01); A23J 1/02 (2006.01); A23J 3/04 (2006.01); A23J 3/22 (2006.01); A23J 3/34 (2006.01); A23K 10/14 (2016.01); A23K 10/20 (2016.01); A23K 20/147 (2016.01); A23K 30/20 (2016.01); A23K 50/40 (2016.01); A23K 50/80 (2016.01); A23L 27/21 (2016.01); A61K 9/00 (2006.01)
CPC A23K 10/20 (2016.05) [A23J 1/02 (2013.01); A23J 3/04 (2013.01); A23J 3/227 (2013.01); A23J 3/341 (2013.01); A23K 10/14 (2016.05); A23K 20/147 (2016.05); A23K 30/20 (2016.05); A23K 50/40 (2016.05); A23K 50/80 (2016.05); A23L 27/215 (2016.08); A61K 9/0056 (2013.01); A61K 35/64 (2013.01)] 12 Claims
 
1. A method for producing Maillard reaction products of an enzymatically hydrolysed water-soluble insect protein, the method comprising the steps of:
a) providing an aqueous water-soluble insect protein composition comprising at least one water-soluble protein, preferably more than one water-soluble protein, wherein the pH of said composition is between pH 4 and pH 8 and wherein the at least one water-soluble protein in said water-soluble protein composition is solubilized, and further providing at least one peptidase;
b) mixing the at least one peptidase of step a) with the aqueous water-soluble insect protein composition of step a), therewith providing a protein/peptidase mixture;
c) heating the protein/peptidase mixture of step b) at a temperature of below 75° C. such that the at least one water-soluble protein in the protein/peptidase mixture is enzymatically hydrolysed by the at least one peptidase, therewith providing enzymatically hydrolysed protein/peptidase solution; and
d) terminating the enzymatic hydrolysis in the hydrolysed protein/peptidase solution of step c) by heating the hydrolysed protein/peptidase solution at a temperature of between 75° C. and 110° C., such that the at least one peptidase is heat-inactivated by the heating, therewith providing the enzymatically hydrolysed water-soluble insect protein;
wherein after step d) in a further step e) the enzymatically hydrolysed water-soluble insect protein of step d) is subjected to a Maillard reaction for the provision of modified amino groups in said enzymatically hydrolysed protein, the Maillard reaction comprising the sub-steps:
e1) providing a carbohydrate and mixing the carbohydrate with the enzymatically hydrolysed water-soluble insect protein of step d), therewith providing an enzymatically hydrolysed insect protein/added carbohydrate mixture;
e2) heating the enzymatically hydrolysed insect protein/added carbohydrate mixture of step e1), therewith providing Maillard reaction products of enzymatically hydrolysed insect protein.