US 11,994,528 B2
Systems, methods, and devices for automated nucleic acid and protein isolation
Alexandre Vlassov, Austin, TX (US); Timothy Barta, Austin, TX (US); Justin Inslee, Broomfield, CO (US); Ethan Gentert, Longmont, CO (US); Robert Smith, Louisville, CO (US); Sara Thiele, Lafayette, CO (US); Carolyn Adams, Boulder, CO (US); Kraig Kruger, Boulder, CO (US); Robert Schneider, Erie, CO (US); Wade Lukianow, Boulder, CO (US); Jay Greco, Arvada, CO (US); Jason Jones, Erie, CO (US); David Cerrone, Broomfield, CO (US); Sumonto Ghosh, Longmont, CO (US); Brian Vajda, Boulder, CO (US); Andrew Basta, Erie, CO (US); Allan Evans, Golden, CO (US); Christopher Crowley, Golden, CO (US); Robert Setterquist, Austin, TX (US); Anne Cox, Golden, CO (US); and Mark Mayernick, Lafayette, CO (US)
Assigned to LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed by LIFE TECHNOLOGIES CORPORATION, Carlsbad, CA (US)
Filed on Dec. 15, 2020, as Appl. No. 17/122,324.
Claims priority of provisional application 62/949,917, filed on Dec. 18, 2019.
Prior Publication US 2021/0190809 A1, Jun. 24, 2021
Int. Cl. G01N 35/10 (2006.01); B01D 61/58 (2006.01); B01D 69/02 (2006.01); G01N 1/40 (2006.01); G01N 21/59 (2006.01); G01N 35/00 (2006.01)
CPC G01N 35/1016 (2013.01) [B01D 61/58 (2013.01); B01D 69/02 (2013.01); G01N 1/405 (2013.01); G01N 1/4077 (2013.01); G01N 21/59 (2013.01); G01N 35/00584 (2013.01); G01N 35/1081 (2013.01); B01D 2311/2642 (2013.01); B01D 2313/44 (2013.01); B01D 2313/50 (2013.01); B01D 2313/58 (2013.01); B01D 2319/025 (2013.01); B01D 2325/42 (2013.01); G01N 2001/4088 (2013.01); G01N 2035/1025 (2013.01); G01N 2035/1053 (2013.01)] 18 Claims
OG exemplary drawing
 
1. An instrument for automated purification of a target biomolecule from a biological sample, comprising:
a purification cartridge comprising an apparatus that includes:
an input reservoir for receiving the biological sample;
a first bioprocessing assembly in fluid communication with the input reservoir and a lysis buffer reservoir, the first bioprocessing assembly configured to generate a lysate comprising the target biomolecule;
a second bioprocessing assembly in fluid communication with the first bioprocessing assembly and a first elution buffer reservoir, the second bioprocessing assembly including a target-biomolecule-binding filter configured to retain the target biomolecule; and
a receptacle in fluid communication with the second bioprocessing assembly, the receptacle configured to receive an output container for receiving the target biomolecule from the second bioprocessing assembly,
the purification cartridge further comprising:
a first external layer comprising a first side and a second side, and
a second external layer disposed opposite the first side of the first external layer;
a casing having an internal compartment configured in size and shape for receiving the purification cartridge;
wherein the internal compartment comprises a position sensor for determining that the purification cartridge has been fully inserted within the internal compartment;
optionally, a selectively closable access door providing access to the internal compartment;
a pump assembly disposed within the internal compartment and configured to provide pumping action through peristaltic motion; and
a clamp mechanism disposed within the internal compartment and configured to move between an open position in which the internal compartment is accessible and a closed position in which the clamp mechanism is clamped against an inserted purification cartridge to enable processing of the biological sample, wherein when in the closed position, the clamp mechanism operates to fluidically seal the purification cartridge by exerting the first external layer and the second external layer towards one another.