	US 11,994,520 B2
	Developing lateral flow immunochromatography (LFIA) peptide-based test strips for rapid detection of antigens and antibodies against specific antigens
Charlotte A. E. Hauser, Thuwal (SA); Panagiotis Bilalis, Thuwal (SA); Dana Alhattab, Thuwal (SA); Hepi Hari Susapto, Thuwal (SA); Manola Moretti, Thuwal (SA); Salwa Alshehri, Thuwal (SA); Ali Aldoukhi, Thuwal (SA); Hamed Albalawi, Thuwal (SA); Hattan Boshah, Thuwal (SA); Abdulelah Alrashoudi, Thuwal (SA); Alexander Valle Perez, Thuwal (SA); and Rosario Perez Pedroza, Thuwal (SA)
Assigned to KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY, Thuwal (SA)
Appl. No. 18/038,107
Filed by KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY, Thuwal (SA)
PCT Filed Nov. 22, 2021, PCT No. PCT/IB2021/060795 § 371(c)(1), (2) Date May 22, 2023, PCT Pub. No. WO2022/107089, PCT Pub. Date May 27, 2022.
Claims priority of provisional application 63/117,138, filed on Nov. 23, 2020.
Prior Publication US 2023/0408518 A1, Dec. 21, 2023
Int. Cl. C07K 14/005 (2006.01); C07K 14/08 (2006.01); G01N 33/543 (2006.01); G01N 33/549 (2006.01); G01N 33/558 (2006.01); G01N 33/569 (2006.01)

CPC G01N 33/56983 (2013.01) [C07K 14/005 (2013.01); G01N 33/54388 (2021.08); G01N 33/558 (2013.01); C07K 2319/00 (2013.01); G01N 2469/20 (2013.01)]

19 Claims

1. A method of fabricating a lateral flow immunoassay (LFIA) comprising:

synthesizing and screening at least one peptide antigen;

conjugating the peptide antigen with at least one tag;

printing a test strip;

determining the minimum thickness of a test cassette; and

assembling the LFIA,

wherein the peptide antigen binds specifically to at least one analyst,

wherein at least one control line and at least one test line are printed on the test strip, and wherein the material in the test line binds the complex formed by the analyst and the peptide antigen conjugated with the tag, wherein the epitopes are at least one selected from the group consisting of peptides with amino acid sequences provided in SEQ ID Nos. 1-10 and 10-19.