US 11,994,468 B2
Fluorescence intensity correcting method, fluorescence intensity calculating method, and fluorescence intensity calculating apparatus
Yasunobu Kato, Kanagawa (JP); and Yoshitsugu Sakai, Kanagawa (JP)
Assigned to Sony Corporation, Tokyo (JP)
Filed by Sony Corporation, Tokyo (JP)
Filed on Apr. 29, 2022, as Appl. No. 17/732,681.
Application 17/732,681 is a continuation of application No. 16/848,115, filed on Apr. 14, 2020, granted, now 11,340,167.
Application 16/848,115 is a continuation of application No. 16/295,519, filed on Mar. 7, 2019, granted, now 10,656,090, issued on May 19, 2020.
Application 16/295,519 is a continuation of application No. 14/452,085, filed on Aug. 5, 2014, granted, now 10,295,466, issued on May 21, 2019.
Application 14/452,085 is a continuation of application No. 13/089,961, filed on Apr. 19, 2011, granted, now 8,825,431, issued on Sep. 2, 2014.
Claims priority of application No. 2010-104566 (JP), filed on Apr. 28, 2010.
Prior Publication US 2022/0260491 A1, Aug. 18, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 21/64 (2006.01)
CPC G01N 21/6428 (2013.01) [G01N 2021/6421 (2013.01); G01N 2021/6439 (2013.01)] 30 Claims
OG exemplary drawing
 
1. A flow cytometer system comprising:
a plurality of detectors configured to receive a light from each of microparticles labeled with a plurality of fluorescent dyes, and the detectors corresponding to different received light wavelength bands;
a plurality of laser light sources configured to radiate laser beams through a flow cell where the microparticles flow; and
a processor circuitry configured to calculate fluorescence intensity for each of the fluorescent dyes using a linear sum of single-dyeing spectra, wherein a number of single-dyeing spectra being equal or less than a number of the detectors, and output a plot diagram of the calculated fluorescence intensity,
wherein the laser light sources include at least one of a 488 nm laser or a 640 nm laser.