US 11,993,807 B2
Affinity reagents having enhanced binding and detection characteristics
Tural Aksel, Redwood City, CA (US); Torri Rinker, San Francisco, CA (US); Markus Burns, Palo Alto, CA (US); Michael Dorwart, San Jose, CA (US); Rachel Galimidi, Belmont, CA (US); Dmitriy Gremyachinskiy, San Diego, CA (US); Stephen Hendricks, Los Gatos, CA (US); Elvis Ikwa, San Leandro, CA (US); Gregory Kapp, San Carlos, CA (US); Joshua Simon Klein, Pacifica, CA (US); Julia Robinson, East Palo Alto, CA (US); Cassandra Stawicki, Foster City, CA (US); Sonal Tonapi, Santa Clara, CA (US); and Parag Mallick, San Mateo, CA (US)
Assigned to Nautilus Subsidiary, Inc., Seattle, WA (US)
Filed by Nautilus Subsidiary, Inc., Seattle, WA (US)
Filed on Mar. 7, 2023, as Appl. No. 18/179,924.
Application 18/179,924 is a continuation of application No. 17/523,869, filed on Nov. 10, 2021, granted, now 11,692,217.
Claims priority of provisional application 63/227,080, filed on Jul. 29, 2021.
Claims priority of provisional application 63/132,170, filed on Dec. 30, 2020.
Claims priority of provisional application 63/112,607, filed on Nov. 11, 2020.
Prior Publication US 2023/0272459 A1, Aug. 31, 2023
Int. Cl. C12Q 1/6832 (2018.01); C12Q 1/6834 (2018.01); C12Q 1/6876 (2018.01)
CPC C12Q 1/6832 (2013.01) [C12Q 1/6834 (2013.01); C12Q 1/6876 (2013.01)] 23 Claims
 
1. A method of detecting an analyte, comprising:
(a) contacting an array of analytes with a detectable probe, wherein the array of analytes comprises individual analytes each attached to sites that are physically isolated from all other analytes in the array of analytes,
wherein the detectable probe comprises:
(i) a retaining component comprising nucleic acid origami,
(ii) two or more label components attached to the retaining component, and
(iii) two or more binding components attached to the retaining component, wherein the two or more binding components recognize the same epitope of the analyte,
wherein the detectable probe binds to the analyte via non-covalent binding of at least one of the two or more binding components to the epitope; and
(b) acquiring signals from the two or more label components of the detectable probe bound to the analyte, thereby detecting the analyte.