CPC C12Q 1/6806 (2013.01) [C12N 15/1093 (2013.01); C12Q 1/6874 (2013.01)] | 9 Claims |
1. An adaptor template oligonucleotide (ATO) for extending polynucleotides comprising:
(a) a 3′ random sequence of 3 to 36 ‘N’ bases;
(b) a 3′ end with a blocker, which renders ATO non-extendible;
(c) a universal sequence, 5′ to the random sequence;
(d) modified nucleotides or linkages which render the ATO resistant to 3′ exonuclease cleavage; and
(e) a uracil nucleotide which renders the ATO degradable.
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