US 11,993,805 B2
Methods, compositions, and kits for preparing nucleic acid libraries
Guoliang Fu, Oxfordshire (GB); and Thomas Dunwell, Oxfordshire (GB)
Assigned to Genefirst Ltd., Oxfordshire (GB)
Filed by Genefirst Ltd., Oxfordshire (GB)
Filed on Jul. 20, 2022, as Appl. No. 17/869,595.
Application 17/869,595 is a division of application No. 16/605,520, granted, now 11,408,025, previously published as PCT/GB2018/051000, filed on Apr. 17, 2018.
Claims priority of application No. 1706059 (GB), filed on Apr. 17, 2017; application No. 1708384 (GB), filed on May 25, 2017; and application No. 1719114 (GB), filed on Nov. 17, 2017.
Prior Publication US 2023/0121409 A1, Apr. 20, 2023
Int. Cl. C12Q 1/68 (2018.01); C12N 15/10 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6874 (2018.01)
CPC C12Q 1/6806 (2013.01) [C12N 15/1093 (2013.01); C12Q 1/6874 (2013.01)] 9 Claims
 
1. An adaptor template oligonucleotide (ATO) for extending polynucleotides comprising:
(a) a 3′ random sequence of 3 to 36 ‘N’ bases;
(b) a 3′ end with a blocker, which renders ATO non-extendible;
(c) a universal sequence, 5′ to the random sequence;
(d) modified nucleotides or linkages which render the ATO resistant to 3′ exonuclease cleavage; and
(e) a uracil nucleotide which renders the ATO degradable.