	US 11,993,802 B2
	Method for stabilizing proteins
Mohsan Waseem Khan, Watford (GB); and Alison Porter, Charvil (GB)
Assigned to LONZA LTD, Visp (CH)
Filed by LONZA LTD, Visp (CH)
Filed on Apr. 22, 2020, as Appl. No. 16/855,714.
Application 16/855,714 is a division of application No. 15/619,876, filed on Jun. 12, 2017, granted, now 10,975,409.
Claims priority of provisional application 62/348,595, filed on Jun. 10, 2016.
Prior Publication US 2020/0248223 A1, Aug. 6, 2020
Int. Cl. C12P 21/00 (2006.01); C07K 1/14 (2006.01); C07K 16/00 (2006.01); C12M 1/00 (2006.01); C12M 1/06 (2006.01); C12M 1/34 (2006.01); C12P 21/02 (2006.01); A61K 38/00 (2006.01)

CPC C12P 21/00 (2013.01) [C07K 1/14 (2013.01); C07K 16/00 (2013.01); C12M 27/02 (2013.01); C12M 29/00 (2013.01); C12M 41/12 (2013.01); C12M 41/28 (2013.01); C12M 41/34 (2013.01); C12M 47/10 (2013.01); C12M 47/20 (2013.01); C12P 21/02 (2013.01); A61K 38/00 (2013.01); C07K 2317/14 (2013.01); C07K 2317/56 (2013.01)]

22 Claims

1. A method of producing a stabilized product expressed in

a recombinant host cell, comprising:

a) culturing the cell, wherein an average redox potential of the production culture is about 0 mV to about 20 mV at the beginning of the culturing step, under conditions that maintain and wherein a decrease of the average redox potential of the production culture does not exceed 60 mV from the beginning of the culturing step to the time of harvest of the cell, and

b) harvesting the cell comprising the stabilized product.