CPC C12N 15/1082 (2013.01) [C12Q 1/6869 (2013.01); C12Q 1/6874 (2013.01)] | 17 Claims |
1. A method for preparing a library of template nucleic acids comprising:
(a) contacting a target nucleic acid with a plurality of transposons under conditions such that a portion of the plurality of transposons are inserted into the target nucleic acid, wherein at least one transposon of the plurality of transposons comprises:
a double-stranded first transposase recognition site;
a double-stranded second transposase recognition site;
a barcode disposed therebetween, wherein the barcode comprises:
a first barcode sequence of the at least one transposon;
a second barcode sequence of the at least one transposon; and
a linker disposed between the first and second barcode sequences, wherein the linker comprises a first primer site, a second primer site and a non-amplifiable site disposed between the first and second primer sites; and
(b) amplifying said target nucleic acid using one or both of the first primer site and the second primer site to separate the first barcode sequence from the second barcode sequence.
|