US 11,993,772 B2
Linking sequence reads using paired code tags
Frank J. Steemers, Encinitas, CA (US); Kevin L Gunderson, Encinitas, CA (US); Thomas Royce, San Diego, CA (US); Natasha Pignatelli, Berkeley, CA (US); Igor Goryshin, Madison, WI (US); and Nicholas Caruccio, Madison, WI (US)
Assigned to ILLUMINA, INC., San Diego, CA (US)
Filed by ILLUMINA, INC., San Diego, CA (US)
Filed on Mar. 25, 2019, as Appl. No. 16/363,874.
Application 16/363,874 is a division of application No. 15/159,588, filed on May 19, 2016, granted, now 10,246,705.
Application 15/159,588 is a continuation of application No. 14/726,309, filed on May 29, 2015, abandoned.
Application 14/726,309 is a continuation of application No. 13/080,345, filed on Apr. 5, 2011, granted, now 9,074,251, issued on Jul. 7, 2015.
Application 13/080,345 is a continuation in part of application No. 13/025,022, filed on Feb. 10, 2011, granted, now 8,829,171.
Prior Publication US 2019/0276821 A1, Sep. 12, 2019
Int. Cl. C12N 15/10 (2006.01); C12Q 1/6869 (2018.01); C12Q 1/6874 (2018.01)
CPC C12N 15/1082 (2013.01) [C12Q 1/6869 (2013.01); C12Q 1/6874 (2013.01)] 17 Claims
 
1. A method for preparing a library of template nucleic acids comprising:
(a) contacting a target nucleic acid with a plurality of transposons under conditions such that a portion of the plurality of transposons are inserted into the target nucleic acid, wherein at least one transposon of the plurality of transposons comprises:
a double-stranded first transposase recognition site;
a double-stranded second transposase recognition site;
a barcode disposed therebetween, wherein the barcode comprises:
a first barcode sequence of the at least one transposon;
a second barcode sequence of the at least one transposon; and
a linker disposed between the first and second barcode sequences, wherein the linker comprises a first primer site, a second primer site and a non-amplifiable site disposed between the first and second primer sites; and
(b) amplifying said target nucleic acid using one or both of the first primer site and the second primer site to separate the first barcode sequence from the second barcode sequence.