US 11,993,769 B2
Compostable silica encapsulation of oligonucleotides for long-term storage
Bichlien Hoang Nguyen, Seattle, WA (US); Karin Strauss, Seattle, WA (US); Robert Grass, Zurich (CH); Jan Wendelin Stark, Langenthal (CH); and Julian Bernhard Koch, Zurich (CH)
Assigned to MICROSOFT TECHNOLOGY LICENSING, LLC, Redmond, WA (US)
Filed by MICROSOFT TECHNOLOGY LICENSING, LLC, Redmond, WA (US)
Filed on Apr. 14, 2021, as Appl. No. 17/230,849.
Prior Publication US 2022/0333095 A1, Oct. 20, 2022
Int. Cl. C12N 15/10 (2006.01)
CPC C12N 15/1006 (2013.01) 20 Claims
OG exemplary drawing
 
1. A particle comprising:
a porous, positively charged core comprising a blend of bis(triethoxysilyl)ethylene and bis(3-triethoxysilylpropyl) disulfide (BTSPD);
an oligonucleotide layer adsorbed to the positive charged core;
an interacting layer comprising polyvinylpyrrolidone (PVP) adsorbed to the oligonucleotide layer; and
an outermost layer of non-porous, hydrolyzed organosilicon disulfide, wherein a diameter of the particle is about 440 nm.