	US 11,993,767 B2
	Method for producing 3D, biocompatible polymer scaffold with a cell-filled cavity
Lutz Kloke, Berlin (DE); Alexander Thomas, Berlin (DE); and Tobias Lam, Berlin (DE)
Assigned to CELLBRICKS GmbH, Berlin (DE)
Appl. No. 17/779,100
Filed by CELLBRICKS GmbH, Berlin (DE)
PCT Filed Nov. 20, 2020, PCT No. PCT/DE2020/100986 § 371(c)(1), (2) Date Nov. 18, 2022, PCT Pub. No. WO2021/104571, PCT Pub. Date Jun. 3, 2021.
Claims priority of application No. 102019132214.6 (DE), filed on Nov. 27, 2019.
Prior Publication US 2023/0133963 A1, May 4, 2023
Int. Cl. C12M 1/12 (2006.01); B29C 64/124 (2017.01); B33Y 10/00 (2015.01); B33Y 70/00 (2020.01); B33Y 80/00 (2015.01); C12N 5/00 (2006.01)

CPC C12M 25/14 (2013.01) [B29C 64/124 (2017.08); B33Y 10/00 (2014.12); B33Y 70/00 (2014.12); B33Y 80/00 (2014.12); C12N 5/0068 (2013.01); C12N 2513/00 (2013.01); C12N 2537/10 (2013.01)]

17 Claims

1. A method including:

(a) constructing a 3D scaffold comprising a biocompatible polymer using a lithographic 3D printing method, the 3D scaffold having an at least partially covered cavity and wherein the biocompatible polymer is obtained by rendering a biopolymer as a starting substance photopolymerizable or photocrosslinkable by introducing a photoreactive group, the biopolymer being selected from a group consisting of proteins, DNA, RNA, carbohydrates, carbohydrate derivatives, collagens, fibrins, alginates, gelatins, and hyaluronic acids; and

(b) filling the at least partially covered cavity with a suspension containing biological cells to colonize the 3D scaffold.