	US 11,993,635 B2
	Genetically encoded system for constructing and detecting biologically active agents
Jerome Fox, Boulder, CO (US); Ankur Sarkar, Boulder, CO (US); Akarawin Hongdusit, Boulder, CO (US); and Edward Kim, Oakland, CA (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY CORPORATE, Denver, CO (US)
Filed by The Regents of the University of Colorado, a Body Corporate, Denver, CO (US)
Filed on Aug. 2, 2022, as Appl. No. 17/816,937.
Application 17/816,937 is a division of application No. 17/141,321, filed on Jan. 5, 2021, granted, now 11,472,847.
Application 17/141,321 is a continuation of application No. PCT/US2019/040896, filed on Jul. 8, 2019.
Claims priority of provisional application 62/694,838, filed on Jul. 6, 2018.
Prior Publication US 2023/0151062 A1, May 18, 2023
Int. Cl. C12N 1/20 (2006.01); C07K 14/005 (2006.01); C07K 14/47 (2006.01)

CPC C07K 14/005 (2013.01) [C07K 14/47 (2013.01); C12N 1/20 (2013.01); C07K 2319/80 (2013.01); C12Y 205/01001 (2013.01); C12Y 205/0101 (2013.01); C12Y 207/01036 (2013.01); C12Y 207/04002 (2013.01); C12Y 301/01048 (2013.01); C12Y 401/01033 (2013.01); C12Y 402/03017 (2013.01); C12Y 402/03018 (2013.01); C12Y 402/03024 (2013.01); C12Y 402/03056 (2013.01); C12Y 503/03002 (2013.01)]

20 Claims

1. A system, comprising;

a first region of DNA that encodes:

(i) a first fusion protein, wherein the first fusion protein comprises a substrate recognition domain coupled to a DNA-binding protein;

(ii) a second fusion protein, wherein the second fusion protein comprises a substrate domain coupled to a subunit of RNA polymerase;

(iii) a protein kinase;

and

(iv) a protein phosphatase; and

a second region of DNA that encodes:

(i) an operator for the DNA-binding protein;

(ii) a binding site for the RNA polymerase operatively coupled to the operator for the DNA-binding protein, wherein the subunit of the RNA polymerase is configured to recruit the RNA polymerase to the binding site; and

(iii) a reporter gene encoding a reporter protein, wherein transcription of the reporter gene is initiated upon (1) binding the DNA-binding protein to the operator and (2) binding the RNA polymerase to the RNA polymerase binding site.