| CPC A61L 27/54 (2013.01) [A61K 48/00 (2013.01); A61L 27/12 (2013.01); A61L 27/18 (2013.01); A61L 27/3608 (2013.01); A61L 27/46 (2013.01); C12Q 1/6834 (2013.01); A61L 2300/414 (2013.01); A61L 2430/02 (2013.01)] | 11 Claims |
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1. A gene-activated material comprising (i) a solid scaffold comprising a first portion of a predetermined dose of at least one nucleic acid; and (ii) a surface of the solid scaffold comprising a second portion of the predetermined dose of the at least one nucleic acid physically bound to said surface;
wherein the first and second portion together comprise the predetermined dose;
wherein the solid scaffold incorporating the at least one nucleic acid is produced by admixing in solution the at least one nucleic acid with scaffolding materials to produce a primary complex containing nucleic acids that are chemically-bound to the scaffolding materials and that are not removed by washing in a 0.9% solution of NaCl, and then, without washing, lyophilizing the admixture, thereby producing a gene-activated material containing a first-chemically bound fraction of the nucleic acid that is incorporated into the scaffold, which is not removed by washing in a 0.9% solution of NaCl, and a second unbound fraction of the nucleic acid that can be removed from the solid scaffold by washing in a 0.9% solution of NaCl;
wherein the gene-activated material is produced by admixing the predetermined dose of the at least one nucleic acid with scaffolding materials in the presence of beta tri-calcium phosphate to produce a primary complex containing the first portion of the dose of the at least one nucleic acid; and lyophilizing or drying the solution used to prepare the scaffold, thereby producing said gene-activated material;
wherein the scaffolding materials comprise beta tri-calcium phosphate and a complexing agent; and
wherein the at least one nucleic acid is DNA.
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