(a) disposing the aquatic organism between a lower fixing unit fixed to a lower end inside a chamber and an upper fixing unit which is spaced apart from the lower fixing unit and is movable in a direction of the lower fixing unit or in an opposite direction thereof so that the aquatic organism is immersed in a culture fluid to which a compound for inducing an individual response is added, adhering the upper fixing unit to the aquatic organism to measure a height of the aquatic organism, and then adjusting a position of the upper fixing unit to fix the aquatic organism;

(b) continuously measuring a digital hologram for the aquatic organism; and

(c) restoring the digital hologram obtained by the measurement to calculate a heart rate change, and determining therefrom whether the individual response is caused by the compound for inducing an individual response,

wherein an algorithm for restoration further includes displaying a 2D-time-resolved relative phase map in real time and storing the heart rate at the same time, and

wherein the heart rate is obtained by an analysis method including:

a) selecting pixels corresponding to a heart area in a secured digital hologram;

b) applying a Fourier spectrum method to each refreshing interval;

c) searching a most frequent heart rate in each refreshing interval; and

d) calculating a heart rate per minute from the most frequent heart rate.