US 12,313,828 B2
Method and apparatus for analysis of a sample by light sheet microscopy
Manuel Amthor, Jena (DE); Daniel Haase, Zöllnitz (DE); Thomas Kalkbrenner, Jena (DE); and Jörg Siebenmorgen, Jena (DE)
Assigned to Carl Zeiss Microscopy GmbH, Jena (DE)
Filed by Carl Zeiss Microscopy GmbH, Jena (DE)
Filed on Feb. 25, 2022, as Appl. No. 17/680,695.
Claims priority of application No. 10 2021 104 871.0 (DE), filed on Mar. 1, 2021.
Prior Publication US 2022/0276478 A1, Sep. 1, 2022
Int. Cl. G02B 21/00 (2006.01); G02B 21/24 (2006.01); G02B 21/36 (2006.01); G06T 7/00 (2017.01)
CPC G02B 21/0032 (2013.01) [G02B 21/244 (2013.01); G02B 21/361 (2013.01); G02B 21/367 (2013.01); G06T 7/0002 (2013.01); G06T 2207/10056 (2013.01); G06T 2207/20084 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for light sheet microscopic examination of a specimen, comprising:
illuminating the specimen with a light sheet in a light sheet plane via an illumination objective;
imaging light emitted by the specimen onto an area-type detector via a detection objective, wherein an optical axis of the detection objective encloses with an optical axis of the illumination objective an angle that differs from 0° and from 180° and intersects the light sheet plane;
recording at least one image with the area-type detector;
analyzing the at least one image using a neural network to determine:
i. whether or not the light sheet plane is located at least partially in a focal plane of the detection objective, and/or
ii. in which direction along the optical axis of the detection objective the light sheet plane is located from the focal plane, and/or
iii. at what distance, measured along the optical axis of the detection objective, the light sheet plane is located from the focal plane.