US 12,313,639 B2
Particle confirming method, particle trapping chip, and particle analyzing system
Hiroyuki Hosokawa, Kanagawa (JP); Tasuku Yotoriyama, Tokyo (JP); Shin Masuhara, Tokyo (JP); and Isamu Nakao, Kanagawa (JP)
Assigned to Sony Corporation, Tokyo (JP)
Appl. No. 16/970,307
Filed by Sony Corporation, Tokyo (JP)
PCT Filed Nov. 11, 2019, PCT No. PCT/JP2019/044100
§ 371(c)(1), (2) Date Aug. 14, 2020,
PCT Pub. No. WO2020/129462, PCT Pub. Date Jun. 25, 2020.
Claims priority of application No. 2018-240079 (JP), filed on Dec. 21, 2018.
Prior Publication US 2021/0364410 A1, Nov. 25, 2021
Int. Cl. C12Q 1/6813 (2018.01); G01N 15/14 (2024.01); G01N 15/1429 (2024.01); G01N 35/00 (2006.01); G06K 9/32 (2006.01); G06K 9/46 (2006.01); G06K 9/62 (2022.01); G06V 20/69 (2022.01)
CPC G01N 35/00584 (2013.01) [C12Q 1/6813 (2013.01); G01N 15/1429 (2013.01); G06V 20/693 (2022.01); G06V 20/695 (2022.01)] 14 Claims
OG exemplary drawing
 
1. A particle confirming method comprising:
a trapping step of trapping a particle through a linker fixed to a well in a plurality of wells in a particle trapping region, comprising:
a labeling step of labeling the particle trapped in the well to impart identification information to the particle, comprising:
coupling a photodegradable linker to the well;
coupling a first fluorescent substance and a second fluorescent substance to a label element holding substance;
coupling the label element holding substance to the linker fixed to the well;
adding a particle to the well;
irradiating the well with excitation light, wherein after being irradiated with excitation light the first fluorescent substance extinguishes and attaches to the particle and the second fluorescent substance extinguishes more slowly than the first substance and the second fluorescent substance is coupled to the particle to provide a label element and impart identification information to the particle;
a correlating step of correlating identification information possessed by the particle trapped in the well in the particle trapping region with position information of the well;
a discharging step of discharging the particle from the well after the correlating step;
an identification information acquiring step of acquiring identification information of the particle after the discharging step; and
a confirming step of confirming whether the particle has been trapped in the well possessing the position information after the discharging step, comprising:
comparing the acquired identification information of the particle after the discharging step with the position information of the well;
if the acquired identification information of the particle after the discharging step matches the position information of the well,
confirming that the particle had been trapped in the well.