US 12,313,623 B2
Blood separation and analysis device and methods
Jeffrey Sutton, Sudbury (CA)
Assigned to 1866402 Ontario Limited, Sudbury (CA)
Appl. No. 17/421,345
Filed by 1866402 ONTARIO LIMITED, Sudbury (CA)
PCT Filed Jan. 7, 2020, PCT No. PCT/CA2020/050014
§ 371(c)(1), (2) Date Jul. 7, 2021,
PCT Pub. No. WO2020/142840, PCT Pub. Date Jul. 16, 2020.
Claims priority of provisional application 62/789,357, filed on Jan. 7, 2019.
Prior Publication US 2022/0065768 A1, Mar. 3, 2022
Int. Cl. G01N 15/06 (2024.01); B01L 3/00 (2006.01); C12Q 1/44 (2006.01); C12Q 1/50 (2006.01); G01N 1/38 (2006.01); G01N 1/40 (2006.01); G01N 33/50 (2006.01); G01N 33/70 (2006.01); G01N 15/01 (2024.01); G01N 15/075 (2024.01)
CPC G01N 33/5002 (2013.01) [B01L 3/502 (2013.01); B01L 3/502715 (2013.01); B01L 3/527 (2013.01); C12Q 1/44 (2013.01); C12Q 1/50 (2013.01); G01N 1/38 (2013.01); G01N 1/4077 (2013.01); G01N 15/06 (2013.01); G01N 33/70 (2013.01); B01L 2200/025 (2013.01); B01L 2200/026 (2013.01); B01L 2200/0631 (2013.01); B01L 2200/0652 (2013.01); B01L 2200/0684 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/16 (2013.01); B01L 2300/042 (2013.01); B01L 2300/047 (2013.01); B01L 2300/0609 (2013.01); B01L 2300/0832 (2013.01); G01N 2001/386 (2013.01); G01N 2001/4083 (2013.01); G01N 15/01 (2024.01); G01N 15/075 (2024.01)] 9 Claims
 
1. A method for determining the amount of an analyte of interest in an unknown quantity of sample comprising:
providing three reaction channels each containing a reagent that reacts with the analyte to produce either a first detectable signal proportional to the amount of the analyte in the reaction channel or a reaction product that can be further reacted to produce the first detectable signal proportional to the amount of the analyte in the reaction channel,
the first reaction channel containing a known first quantity of the analyte of interest n,
the second reaction channel containing a known second quantity of the analyte of interest m, wherein m is different than n,
and the third reaction channel containing a known third quantity of the analyte of interest o, wherein o≠m or n,
depositing a volume of the sample in each of the reaction channels;
measuring the first detectable signal produced in each reaction channel; and
determining the amount of analyte in the unknown sample based on the method of standard addition.