| CPC G01N 33/24 (2013.01) [A01B 79/005 (2013.01); B01L 3/50273 (2013.01); B01L 3/502738 (2013.01); F04B 19/006 (2013.01); B01L 2200/0605 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0877 (2013.01); B01L 2300/0883 (2013.01); B01L 2300/0887 (2013.01); F04B 13/02 (2013.01); F04B 43/073 (2013.01); F16K 99/0015 (2013.01); F16K 99/0059 (2013.01); F16K 2099/0084 (2013.01); G01N 33/245 (2024.05)] | 17 Claims |

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1. A microfluidic substrate for processing an agricultural sample slurry for analysis comprising:
a substrate body comprised of a plurality of polymeric planar layers coupled together;
the layers comprising in order of assembly:
a first outer layer including a plurality of liquid fittings and air fittings, the air fittings fluidly coupled to a pressurized air source;
a fluid distribution layer adjacent the first outer layer, the fluid distribution layer comprising a plurality of air distribution microchannels and first liquid distribution micro channels;
an air layer adjacent the fluid distribution layer, the air layer comprising a plurality of air pressure signal ports fluidly coupled to the air distribution microchannels of the fluid distribution layer;
a liquid layer adjacent the air layer, the liquid layer comprising a plurality of second liquid distribution microchannels; and
a second outer layer adjacent the liquid layer;
wherein the air layer and liquid layer codefine a plurality of micropumps at an interface between the air and liquid layers.
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