US 12,313,609 B2
Chromatography method, method of determining the concentration of at least one compound in chromatography method and method of obtaining at least one chromatography method parameter
Jan Schwellenbach, Göttingen (DE); Volkmar Thom, Göttingen (DE); and Dominik Stein, Göttingen (DE)
Assigned to Sartorius Stedim Biotech GmbH, (DE)
Appl. No. 17/611,868
Filed by Sartorius Stedim Biotech GmbH, Göttingen (DE)
PCT Filed May 8, 2020, PCT No. PCT/EP2020/062830
§ 371(c)(1), (2) Date Nov. 16, 2021,
PCT Pub. No. WO2020/239388, PCT Pub. Date Dec. 3, 2020.
Claims priority of application No. 19176462 (EP), filed on May 24, 2019.
Prior Publication US 2022/0214319 A1, Jul. 7, 2022
Int. Cl. G01N 30/88 (2006.01); G01N 30/86 (2006.01)
CPC G01N 30/8658 (2013.01) [G01N 30/88 (2013.01); G01N 2030/8831 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method of determining the concentration of at least one compound in a chromatography method comprising the steps of
selecting the at least one compound;
selecting a stationary phase;
selecting a mobile phase;
selecting a chromatography device having a chromatography bed comprising the stationary phase and the mobile phase;
obtaining an adsorption isotherm of the at least one compound on the stationary phase;
obtaining the maximum diffusion time tdiff of the at least one compound in the mobile phase;
selecting a minimum residence time tres of the at least one compound in the chromatography bed such that Equation I is satisfied
tres>tdiff  Equation I
calculating a concentration c(z, t) of the at least one compound in the mobile phase at a predetermined location z of the chromatography bed and at a predetermined time t based on the adsorption isotherm,
wherein the calculation step is carried out without taking the diffusion of the at least one compound in the mobile phase into account.