US 12,313,509 B2
Sample solution preparation method for measurement by immunity analysis method using fluorescence, measurement cell, measurement kit, and sample solution preparation device
Ayuko Imai, Kawasaki (JP); and Ken Sumiyoshi, Kawasaki (JP)
Assigned to TIANMA JAPAN, LTD., Kanagawa (JP)
Filed by TIANMA JAPAN, LTD., Kanagawa (JP)
Filed on Apr. 2, 2021, as Appl. No. 17/221,231.
Claims priority of application No. 2020-068486 (JP), filed on Apr. 6, 2020.
Prior Publication US 2021/0310912 A1, Oct. 7, 2021
Int. Cl. G01N 1/40 (2006.01); G01N 1/28 (2006.01); G01N 21/64 (2006.01); G01N 33/02 (2006.01); G01N 33/12 (2006.01); G01N 33/53 (2006.01); G01N 33/58 (2006.01)
CPC G01N 1/4005 (2013.01) [G01N 1/286 (2013.01); G01N 21/6428 (2013.01); G01N 33/02 (2013.01); G01N 33/12 (2013.01); G01N 2021/6439 (2013.01)] 10 Claims
OG exemplary drawing
 
10. A sample solution preparation method for measuring a concentration of a target compound contained in food by comparing a degree of fluorescence intensity or fluorescence polarization emitted from a label of the target compound with a previously prepared calibration curve in an immunity analysis method using fluorescence, the sample solution preparation method including:
a dialysis step for bringing a target compound-containing solution that includes the target compound into contact with a dialysis fluid through a dialysis membrane to transfer the target compound to the dialysis fluid, wherein
a molecular weight cut-off of the dialysis membrane is within a range of 2×102 Da to 2×105 Da,
a volume of the dialysis fluid is relatively smaller than a volume of the target compound-containing solution, and a ratio of the volume of the dialysis fluid to the volume of the target compound-containing solution (the volume of the dialysis fluid/the volume of the target compound-containing solution) is within a range of 1/10,000 to 1/10, and
the dialysis step is performed while agitating, with a stirrer chip, at least one of the target compound-containing solution or the dialysis fluid, the stirrer chip including silver-coated iron oxide particles or silver-iron oxide particles.