US 12,312,632 B2
Systems and methods for performing antimicrobial susceptibility testing
Kelly Flentie, Charlestown, MA (US); and Eric Stern, Charlestown, MA (US)
Assigned to SeLux Diagnostics, Inc., Charlestown, MA (US)
Filed by SeLux Diagnostics, Inc., Charlestown, MA (US)
Filed on Nov. 1, 2023, as Appl. No. 18/499,761.
Application 18/499,761 is a continuation of application No. 17/004,848, filed on Aug. 27, 2020, granted, now 11,845,976.
Claims priority of provisional application 62/892,305, filed on Aug. 27, 2019.
Prior Publication US 2024/0102074 A1, Mar. 28, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/18 (2006.01)
CPC C12Q 1/18 (2013.01) 19 Claims
 
1. A method for determining a qualitative susceptibility result of an antimicrobial for a microorganism-comprising sample, the method comprising:
inoculating a plurality of panels comprising a plurality of reservoirs comprising one or more dilution series reservoirs, one or more high microorganism load reservoirs (HMLRs), and one or more control reservoirs, wherein
a microorganism is present at a first concentration Cm0 in the one or more dilution series reservoirs comprising an antimicrobial A that extends from a low concentration of antimicrobial (CAL) that is at or below a susceptible breakpoint to a high concentration of antimicrobial (CAH) that is at or above a resistant breakpoint;
the microorganism is present at a second concentration≥2×Cm0 in the one or more HMLRs comprising the antimicrobial A at a third concentration≥CAL/4; and
the microorganism is not present in one or more control reservoirs comprising the antimicrobial A;
incubating the plurality of panels under conditions promoting microorganism growth;
measuring a relative growth in the one or more dilutions series reservoirs, the one or more HMLRs, and the one or more control reservoirs; and
determining a qualitative susceptibility interpretations from the relative growth measurements.