	US 12,312,622 B2
	Method for producing guanidino acetic acid (GAA)
Frank Schneider, Halle (DE); Kay Marin, Borgholzhausen (DE); Melanie Nickolaus, Spenge (DE); Julia Tegethoff, Halle (DE); and Marleen Oesterhoff, Paderborn (DE)
Assigned to Evonik Operations GmbH, Essen (DE)
Appl. No. 18/870,088
Filed by Evonik Operations GmbH, Essen (DE)
PCT Filed May 24, 2023, PCT No. PCT/EP2023/063902 § 371(c)(1), (2) Date Nov. 27, 2024, PCT Pub. No. WO2023/232584, PCT Pub. Date Dec. 7, 2023.
Claims priority of application No. 22177256 (EP), filed on Jun. 3, 2022.
Prior Publication US 2025/0115938 A1, Apr. 10, 2025
Int. Cl. C12P 13/04 (2006.01); C12N 9/06 (2006.01); C12N 9/10 (2006.01); C12N 9/12 (2006.01); C12N 15/77 (2006.01); C12P 13/10 (2006.01)

CPC C12P 13/04 (2013.01) [C12N 9/0016 (2013.01); C12N 9/1007 (2013.01); C12N 9/1096 (2013.01); C12N 9/1217 (2013.01); C12N 15/77 (2013.01); C12P 13/10 (2013.01); C12Y 201/01002 (2013.01); C12Y 201/04001 (2013.01); C12Y 206/01044 (2013.01); C12Y 207/02002 (2013.01)]

15 Claims

1. A microorganism, comprising:

at least one heterologous gene coding for a protein having a function of a L-arginine:glycine amidinotransferase belonging to E.C. 2.1.4.1;

at least one heterologous gene coding for a protein having a function of a carbamate kinase belonging to E.C. 2.7.2.2; and

further comprising at least one gene coding for a protein having a function of a NADH-dependent amino acid dehydrogenase belonging to E.C. 1.4.1.