US 12,312,595 B2
Hydrogel particles as feeder cells and as synthetic antigen presenting cells
Anh Tuan Nguyen, San Francisco, CA (US); Jeffrey Kim, Berkeley, CA (US); Keunho Ahn, Pleasanton, CA (US); and Daixuan Zhang, Fremont, CA (US)
Assigned to Slingshot Biosciences, Inc., Emeryville, CA (US)
Filed by Slingshot Biosciences, Inc., Emeryville, CA (US)
Filed on Sep. 24, 2024, as Appl. No. 18/894,924.
Application 18/894,924 is a continuation of application No. 18/386,107, filed on Nov. 1, 2023, granted, now 12,134,779.
Application 18/386,107 is a continuation of application No. PCT/US2022/048283, filed on Oct. 28, 2022.
Claims priority of provisional application 63/320,016, filed on Mar. 15, 2022.
Claims priority of provisional application 63/320,009, filed on Mar. 15, 2022.
Claims priority of provisional application 63/274,316, filed on Nov. 1, 2021.
Claims priority of provisional application 63/273,741, filed on Oct. 29, 2021.
Prior Publication US 2025/0011712 A1, Jan. 9, 2025
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/00 (2006.01); A61K 35/17 (2015.01); C12M 1/42 (2006.01); C12N 5/0783 (2010.01)
CPC C12N 5/0018 (2013.01) [A61K 35/17 (2013.01); C12M 35/06 (2013.01); C12N 5/0636 (2013.01); C12N 2500/50 (2013.01); C12N 2501/51 (2013.01); C12N 2501/515 (2013.01)] 27 Claims
OG exemplary drawing
 
1. A method for separating a target cell from an aqueous mixture, the method comprising:
a) incubating an aqueous mixture comprising the target cell and a magnetic capture hydrogel particle, thereby producing a target cell-bound magnetic capture hydrogel particle, wherein the magnetic capture hydrogel particle comprises:
i) a hydrogel matrix comprising a magnetic material embedded and/or encapsulated therein, such that the magnetic capture hydrogel particle is capable of being attracted to or repelled by a magnetic field;
ii) disulfide crosslinks enabling the hydrogel matrix to dissolve upon addition of a reducing agent; and
iii) a ligand capable of binding the hydrogel matrix to the target cell;
b) applying a magnetic field to the incubated aqueous mixture to isolate the target cell-bound magnetic capture hydrogel particle from the aqueous mixture;
c) retrieving the isolated target cell-bound magnetic capture hydrogel particle; and
d) contacting the hydrogel matrix with a reducing agent, thereby dissolving the hydrogel matrix and releasing the target cell from the magnetic capture hydrogel particle.