US 12,312,582 B2
Method for isolation of nucleic acid containing particles and extraction of nucleic acids therefrom
Johan Karl Olov Skog, Lincoln, MA (US); and Leileata M. Russo, New York, NY (US)
Assigned to Exosome Diagnostics, Inc., Waltham, MA (US)
Filed by Exosome Diagnostics, Inc., Waltham, MA (US)
Filed on Mar. 24, 2021, as Appl. No. 17/211,484.
Application 17/211,484 is a continuation of application No. 14/877,531, filed on Oct. 7, 2015, granted, now 10,988,755.
Application 14/877,531 is a continuation of application No. 13/883,673, abandoned, previously published as PCT/US2011/060251, filed on Nov. 10, 2011.
Claims priority of provisional application 61/412,369, filed on Nov. 10, 2010.
Prior Publication US 2021/0207124 A1, Jul. 8, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/10 (2006.01); B01D 21/26 (2006.01)
CPC C12N 15/1003 (2013.01) [B01D 21/26 (2013.01); B01D 21/262 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method for extracting nucleic acids from a biological sample from a subject, comprising the steps of:
a) isolating microvesicles comprising exosomes from the biological sample by one or more centrifugation procedures to pellet the microvesicles from the biological sample and generate isolated, pelleted microvesicles comprising exosomes, wherein none of the centrifugation procedures are performed at a speed exceeding 20,000 g;
and b) extracting nucleic acids from the isolated, pelleted microvesicles comprising exosomes, wherein the extracted nucleic acids comprise 18S and 28S rRNAs.