| CPC C12M 25/04 (2013.01) | 19 Claims |

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1. A tissue culture plate material retainer system comprising:
a) a tissue culture plate comprising a well having an insert opening having an insert opening diameter, a base and a depth from said insert opening to said base;
b) a material configured in the base of said well;
c) material retainer comprising:
i) a retainer ring extending from a first end to a second end;
ii) a retainer ring gap extending a ring gap degree of at least 10 degrees between the first end and the second end of the retainer ring;
wherein the retainer ring has a retainer ring diameter greater than the insert diameter of said well;
wherein the retainer ring gap enables compression of the retainer ring to reduce said retainer ring diameter for insertion of the retainer ring into the insert opening of the well; and
iii) a plurality of retainer legs each extending from a leg connected end, connected to the retainer ring to an extended end of the retainer leg that holds said material against the base of the well; and
wherein at least a portion of the plurality of retainer legs further comprises a lea foot having a leg foot extension that extends radially inward from said extended end of the retainer leg to a foot extended end.
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