US 12,311,370 B2
Microfluidic device for cell characterization
Johan Elf, Uppsala (SE); Ove Öhman, Uppsala (SE); Martin Lovmar, Mölndal (SE); and Özden Baltekin, Uppsala (SE)
Assigned to Astrego Diagnostics AB, Uppsala (SE)
Appl. No. 16/759,203
Filed by ASTREGO DIAGNOSTICS AB, Uppsala (SE)
PCT Filed Oct. 30, 2018, PCT No. PCT/SE2018/051108
§ 371(c)(1), (2) Date Apr. 24, 2020,
PCT Pub. No. WO2019/088904, PCT Pub. Date May 9, 2019.
Claims priority of application No. 1751352-4 (SE), filed on Oct. 31, 2017.
Prior Publication US 2020/0316600 A1, Oct. 8, 2020
Int. Cl. G01N 33/483 (2006.01); B01L 3/00 (2006.01)
CPC B01L 3/502761 (2013.01) [G01N 33/4833 (2013.01); B01L 2200/0652 (2013.01); B01L 2300/0864 (2013.01); B01L 2400/086 (2013.01)] 19 Claims
 
1. A microfluidic device comprising:
a substrate having a flow input channel, a flow output channel and spatially defined and separated cell channels extending from said flow input channel to said flow output channel and substantially parallel to one another, said spatially defined and separated cell channels being configured to accommodate cells,
a respective first end of said spatially defined and separated cell channels being in fluid connection with said flow input channel and a respective second end of said spatially defined and separated cell channels being in fluid connection with said flow output channel; and
at least one identification surface comprising immobilized affinity molecules configured to capture cells of a species or serotype or of a group of species or serotypes, wherein
said spatially defined and separated cell channels comprise a respective obstruction arranged at or in connection with the respective second end of said spatially defined and separated cell channels; and
said respective obstruction is operable to prevent selected cells which enter said respective first end of said spatially defined and separated cell channels from said flow input channel from passing said respective obstruction, out through said respective second end of said spatially defined and separated cell channels, and into said flow output channel, whereby said respective obstruction is operable to trap said selected cells in said spatially defined and separated cell channels, and wherein said spatially defined and separated cell channels are dimensioned to support growth of said selected cells in said spatially defined and separated cell channels.