US 12,311,076 B1
Demineralized bone matrix composition with enhanced osteoinductivity and osteoconductivity
Frank Vizesi, Carlsbad, CA (US); Scott David Boden, Carlsbad, CA (US); Tyler Paul Lipschultz, Carlsbad, CA (US); Michelle Esplago Pacer, Carlsbad, CA (US); and Doo Wan Kim, Carlsbad, CA (US)
Assigned to Seaspine, Inc., Carlsbad, CA (US)
Filed by Seaspine, Inc., Carlsbad, CA (US)
Filed on Jun. 6, 2019, as Appl. No. 16/433,668.
Claims priority of provisional application 62/681,842, filed on Jun. 7, 2018.
Int. Cl. A61L 27/36 (2006.01)
CPC A61L 27/3608 (2013.01) [A61L 27/3691 (2013.01); A61L 2430/02 (2013.01)] 9 Claims
 
1. A method of producing an osteoinductive and osteoconductive composition for in vivo use, the method comprising:
obtaining bone tissue;
cleaning the bone tissue obtained with a plurality of washes;
milling the cleaned bone tissue into a plurality of fibers, wherein each fiber of the plurality of fibers has a geometry selected from:
a long wide fiber, wherein the long wide fiber has a length of about 10 mm or greater and a width between about 0.6 mm and about 1.0 mm,
a long narrow fiber, wherein the long narrow fiber has a length of greater than about 10 mm and a width between about 0.2 mm and about 0.6 mm,
a short wide fiber, wherein the short wide fiber has a length of less than about 10 mm and a width between about 0.6 mm and about 1.0 mm, or
a short narrow fiber, wherein the short narrow fiber has a length of less than about 10 mm and a width between about 0.2 mm and about 0.6 mm;
demineralizing the plurality of fibers to expose one or more biologically active components;
selecting a mixture of fibers from the plurality of fibers, wherein the mixture consists of a ratio of long wide fibers to long narrow fibers;
combining the mixture of fibers to yield an osteoinductive and osteoconductive composition having osteoinductive properties and osteoconductive properties, wherein:
the ratio comprises a larger proportion of long wide fibers to impart greater osteoinductive properties to the osteoinductive and osteoconductive composition, or
the ratio comprises a larger proportion of long narrow fibers to impart greater osteoconductive properties to the osteoinductive and osteoconductive composition.