| CPC A61K 48/0033 (2013.01) [A61K 9/5123 (2013.01); A61K 38/1774 (2013.01); A61K 38/465 (2013.01); A61K 47/60 (2017.08); A61K 47/6849 (2017.08); A61K 47/6889 (2017.08); A61K 47/6929 (2017.08); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/88 (2013.01); C12N 2310/20 (2017.05)] | 30 Claims |

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1. A lipid nanoparticle (LNP) comprising a lipid composition comprising:
about 35 to about 65 mol % an ionizable cationic lipid of structure
![]() wherein R is
![]() about 0.5 to about 3 mol % PEG-lipid comprising functionalized PEG-lipid and non-functionalized PEG-lipid,
about 7 to about 13 mol % a phospholipid, and
about 27 to about 50 mol % a sterol.
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