US 12,311,001 B2
Use of extracellular vesicles of Rhizoma Drynariae in preparation of medicine for treating orthopedic diseases
Kewei Zhao, Guangzhou (CN); and Qing Zhao, Guangzhou (CN)
Assigned to THE THIRD AFFILIATED HOSPITAL OF GUANGZHOU UNIVERSITY OF CHINESE MEDICINE, Guangzhou (CN)
Appl. No. 18/559,889
Filed by THE THIRD AFFILIATED HOSPITAL OF GUANGZHOU UNIVERSITY OF CHINESE MEDICINE, Guangzhou (CN)
PCT Filed Aug. 16, 2022, PCT No. PCT/CN2022/112697
§ 371(c)(1), (2) Date Nov. 9, 2023,
PCT Pub. No. WO2023/155398, PCT Pub. Date Aug. 24, 2023.
Claims priority of application No. 202210141049.3 (CN), filed on Feb. 16, 2022.
Prior Publication US 2025/0000923 A1, Jan. 2, 2025
Int. Cl. A61K 36/00 (2006.01); A61K 36/126 (2006.01); A61K 45/06 (2006.01)
CPC A61K 36/126 (2013.01) [A61K 45/06 (2013.01); A61K 2236/31 (2013.01); A61K 2236/53 (2013.01)] 1 Claim
 
1. A method of making extracellular vesicles of Rhizoma Drynariae comprising the following steps:
(1) juicing fresh Rhizoma Drynariae, filtering, collecting a filtrate, centrifuging to remove impurities and collecting a supernatant; wherein the centrifuging comprises three centrifugations, a first centrifugation performed at a speed of 100-500 g producing a first supernatant, a second centrifugation performed on said supernatant at a speed of 1000-3000 g producing a second supernatant, and a third centrifugation performed at a speed of 8000-12000 g producing a third supernatant; wherein the centrifugations are each independently performed for 5-30 min;
(2) centrifuging the third supernatant at a speed of 100000-200000 g for 60-120 min, and collecting a precipitate to obtain the extracellular vesicles of Rhizoma Drynariae wherein the extracellular vesicles of Rhizoma Drynariae contains naringin, eriodictyol, cinnamic acid, naringenin, prunin, caffeic acid, sinapinic acid, afzelin, secoisolariciresinol, narirutin and kaempferol.