	US 12,310,985 B2
	Composition and method for treatment of diabetes
Taolei Sun, Wuhan (CN)
Assigned to SHENZHEN PROFOUND VIEW PHARMACEUTICAL TECHNOLOGY, Shenzhen (CN)
Appl. No. 17/753,307
Filed by SHENZHEN PROFOUND VIEW PHARMACEUTICAL TECHNOLOGY, Shenzhen (CN)
PCT Filed Oct. 15, 2020, PCT No. PCT/CN2020/121079 § 371(c)(1), (2) Date Feb. 26, 2022, PCT Pub. No. WO2021/073563, PCT Pub. Date Apr. 22, 2021.
Prior Publication US 2022/0273706 A1, Sep. 1, 2022
Int. Cl. A61K 33/242 (2019.01); A61K 47/54 (2017.01); A61K 47/64 (2017.01); A61P 3/10 (2006.01)

CPC A61K 33/242 (2019.01) [A61K 47/542 (2017.08); A61K 47/64 (2017.08); A61P 3/10 (2018.01)]

20 Claims

1. A method for producing ligand-modified gold clusters (AuCs), said method comprising:

providing an HAuCl₄solution;

sequentially adding an acidic solution and a first ligand solution into the HAuCl₄solution to form a first mixture solution; wherein the molar ratio between the first ligand and HAuCl₄is in the range of 1:1 to 20:1;

adding NaBH₄solution into the first mixture solution to form a second mixture solution; wherein the molar ratio between NaBH₄and HAuCl₄is in the range of 1:1 to 10:1;

adding an aprotic polar solvent to the second mixture solution to terminate reaction and to obtain a third mixture solution;

centrifuging the third mixture solution to collect solid sediment;

dissolving the collected solid sediment in a second ligand solution to obtain a fourth mixture solution and maintaining the fourth mixture solution for a period; wherein the molar ratio between the second ligand and HAuCl₄is in the range of 1:1 to 20:1;

centrifuging the fourth mixture solution to obtain supernatant; and

dialyzing the supernatant in a dialysis bag with a predetermined cut-off molecular size.