US 12,310,324 B2
Compositions and methods for minimizing nornicotine synthesis in tobacco
Ralph E. Dewey, Apex, NC (US); and Ramsey S. Lewis, Apex, NC (US)
Assigned to North Carolina State University, Raleigh, NC (US)
Filed by North Carolina State University, Raleigh, NC (US)
Filed on Dec. 21, 2023, as Appl. No. 18/392,210.
Application 18/392,210 is a continuation of application No. 17/653,699, filed on Mar. 7, 2022, granted, now 11,877,556.
Application 17/653,699 is a continuation of application No. 16/868,750, filed on May 7, 2020, granted, now 11,304,395, issued on Apr. 19, 2022.
Application 16/868,750 is a continuation of application No. 15/637,865, filed on Jun. 29, 2017, granted, now 10,681,883, issued on Jun. 16, 2020.
Application 15/637,865 is a continuation of application No. 14/980,523, filed on Dec. 28, 2015, granted, now 10,194,624, issued on Feb. 5, 2019.
Application 14/980,523 is a continuation of application No. 13/521,766, granted, now 9,247,706, issued on Feb. 2, 2016, previously published as PCT/US2011/021088, filed on Jan. 13, 2011.
Claims priority of provisional application 61/295,671, filed on Jan. 15, 2010.
Prior Publication US 2024/0122151 A1, Apr. 18, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A01H 6/82 (2018.01); A01H 5/12 (2018.01); A24B 13/00 (2006.01); C12N 9/02 (2006.01); C12N 15/82 (2006.01); C12Q 1/6895 (2018.01)
CPC A01H 6/823 (2018.05) [A01H 5/12 (2013.01); A24B 13/00 (2013.01); C12N 9/0073 (2013.01); C12N 15/8243 (2013.01); C12Q 1/6895 (2013.01); C12Q 2600/13 (2013.01); C12Q 2600/156 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A Nicotiana tabacum plant comprising a mutant CYP82E10 gene encoding a truncated CYP82E10 polypeptide comprising a mutation compared to SEQ ID NO: 2, wherein said mutant CYP82E10 gene results in reduced expression or activity of said CYP82E10 polypeptide when compared to the activity of a wild-type CYP82E10 polypeptide under the same test conditions.