US 11,990,228 B2
Automatic assay assessment and normalization for image processing
Yao Nie, Sunnyvale, CA (US); and Maria V. Sainz De Cea, Chicago, IL (US)
Assigned to VENTANA MEDICAL SYSTEMS, INC., Tucson, AZ (US)
Filed by Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed on May 31, 2023, as Appl. No. 18/326,716.
Application 18/326,716 is a continuation of application No. 17/827,656, filed on May 27, 2022, granted, now 11,715,557.
Application 17/827,656 is a continuation of application No. 16/777,649, filed on Jan. 30, 2020, granted, now 11,380,085, issued on Jul. 5, 2022.
Application 16/777,649 is a continuation of application No. PCT/EP2018/070978, filed on Aug. 2, 2018.
Claims priority of provisional application 62/541,621, filed on Aug. 4, 2017.
Prior Publication US 2023/0317253 A1, Oct. 5, 2023
Int. Cl. G06T 3/40 (2024.01); G01N 15/1433 (2024.01); G06F 16/535 (2019.01); G06F 18/214 (2023.01); G06V 20/69 (2022.01); G16H 10/40 (2018.01); G16H 30/40 (2018.01); G16H 50/20 (2018.01); G16H 70/60 (2018.01); G01N 15/10 (2006.01)
CPC G16H 30/40 (2018.01) [G01N 15/1433 (2024.01); G06F 16/535 (2019.01); G06F 18/214 (2023.01); G06T 3/40 (2013.01); G06V 20/698 (2022.01); G16H 10/40 (2018.01); G16H 50/20 (2018.01); G16H 70/60 (2018.01); G01N 2015/1006 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of normalizing a titer of a stain within a query image to a titer of a stain in a template image, comprising:
acquiring the query image, the query image being of a biological sample stained with the stain;
estimating the titer of the stain within the query image;
determining that the estimated titer of the stain is within a predetermined threshold titer range; and
in response to determining that the estimated titer of the stain is within the predetermined threshold titer range, normalizing the titer of the stain within the query image by reconstructing an RGB image from the query image, wherein the RGB image is reconstructed based on a color model that incorporates density information.