US 11,990,184 B2
DNA backbone editing for DNA data storage
Gemma Mendonsa, Minneapolis, MN (US); Riyan Alex Mendonsa, Minneapolis, MN (US); and Tim Rausch, Farmington, MN (US)
Assigned to SEAGATE TECHNOLOGY LLC, Fremont, CA (US)
Filed by Seagate Technology LLC, Fremont, CA (US)
Filed on Sep. 24, 2020, as Appl. No. 17/031,664.
Prior Publication US 2022/0090157 A1, Mar. 24, 2022
Int. Cl. C12P 19/34 (2006.01); G11C 13/02 (2006.01)
CPC G11C 13/02 (2013.01) [C12P 19/34 (2013.01)] 8 Claims
OG exemplary drawing
 
1. A method of synthesizing a DNA strand encoding data, the method comprising:
cleaving a DNA backbone having multiple PAM sequences into multiple segments, by moving, via voltage, at least one of the DNA backbone and a set of Cas9 proteins combined with a set of gRNAs across a hydrophobic fluidic platform comprising multiple cells each configured to independently receive a voltage, and combining on the platform the DNA backbone and the set of Cas9 proteins combined with the set of gRNAs at a first location on the platform, resulting in the multiple segments each having terminal ends;
moving across the platform to a second location on the platform, via voltage applied to individual cells, a plurality of data-encoding symbols having terminal ends on the platform;
inserting the symbols between the multiple segments using a homology-directed repair (HDR) process, and
joining the terminal ends of the segments to homologous terminal ends of the symbols in the presence of a ligase or a ligase with a polymerase using another homology-directed repair (HDR) process and forming the DNA strand encoding data comprising alternating segments and symbols.