US 11,988,824 B2
Microscope and method for forming a microscopic image with an extended depth of field
Alexander Gaiduk, Jena (DE); Ilja Karanin, Munich (DE); Pavlos Iliopoulos, Weimar (DE); Jin Young Sohn, Fullerton, CA (US); Gyoungil Cho, Fullerton, CA (US); and Cheong Soo Seo, Brea, CA (US)
Assigned to Carl Zeiss Microscopy GmbH, Jena (DE); SD Optics, Inc., Seoul (KR); and Stereo Display, Inc., Anaheim, CA (US)
Filed by Carl Zeiss Microscopy GmbH, Jena (DE); SD Optics, Inc., Seoul (KR); and Stereo Display, Inc., Anaheim, CA (US)
Filed on Mar. 16, 2022, as Appl. No. 17/696,744.
Claims priority of application No. 21163120 (EP), filed on Mar. 17, 2021.
Prior Publication US 2022/0299746 A1, Sep. 22, 2022
Int. Cl. G02B 21/36 (2006.01); H04N 25/531 (2023.01); H04N 25/705 (2023.01)
CPC G02B 21/361 (2013.01) [H04N 25/531 (2023.01); H04N 25/705 (2023.01)] 15 Claims
OG exemplary drawing
 
1. A method for forming a microscopic image with an extended depth of field by means of a microscope that comprises an images sensor, wherein the image sensor comprises pixels that are arranged as a matrix that is formed by lines, wherein the method comprises the following steps:
acquiring a plurality of microscopic frames of a specimen while a focus position (z) is changed, wherein the microscopic frames are acquired line by line, and wherein the focus position (z) is changed over a course of acquiring individuals of the microscopic frames;
identifying parts of individuals of the acquired lines that sharply image the specimen;
composing the identified parts of the lines in order to form a microscopic image of the specimen with an extended depth of field;
recording a value of the focus position for each of the parts of the lines of each of the microscopic frames; and
wherein the composition of the identified parts of the lines in order to form a microscopic image of the specimen with an extended depth of field is based on the recorded values of the focus position (z) assigned to the identified parts of the lines.