US 11,988,675 B2
Methods for quantifying frataxin activity
Joan David Bettoun, Elkins Park, PA (US)
Assigned to Larimar Therapeutics, Inc., Bala Cynwyd, PA (US)
Filed by Larimar Therapeutics, Inc., Bala Cynwyd, PA (US)
Filed on Nov. 25, 2020, as Appl. No. 17/105,149.
Claims priority of provisional application 62/940,214, filed on Nov. 25, 2019.
Prior Publication US 2021/0156874 A1, May 27, 2021
Int. Cl. G01N 33/68 (2006.01); G01N 33/53 (2006.01)
CPC G01N 33/6893 (2013.01) [G01N 33/5306 (2013.01); G01N 2333/902 (2013.01)] 25 Claims
OG exemplary drawing
 
1. A method for measuring enzymatic activity of a Frataxin (FXN) protein, wherein said enzymatic activity comprises enzymatically catalyzed generation of reactive oxygen species (ROS), said method comprising:
(a) combining said FXN protein with a reducing agent and an ROS detector compound, thereby producing an assay mixture;
(b) measuring the amount of ROS generated in the assay mixture over time; and
(c) determining an enzymatic parameter associated with the ROS generating activity of the FXN protein;
wherein said FXN protein facilitates oxidation of the reducing agent and generation of ROS, such that the amount of ROS generated is higher than the amount of ROS generated in the assay mixture comprising the reducing agent and the ROS detector compound without the FXN protein.