| CPC G01N 33/689 (2013.01) [C12N 5/0604 (2013.01); G01N 2800/367 (2013.01); G01N 2800/368 (2013.01)] | 16 Claims |
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1. A method for performing oocyte selection comprising:
obtaining at least two cumulus-oocyte complexes from a donor;
harvesting a cumulus cell mass from each of the at least two cumulus-oocyte complexes, each cumulus cell mass associated with a corresponding oocyte from which the cumulus cell mass was harvested;
measuring an mRNA expression level of each gene of a set of genes from each cumulus cell mass, wherein the set of genes includes amphiregulin (AREG), hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (HSD3B1), luteinizing hormone/choriogonadotropin receptor (LHCGR), and Pappalysin 1 (PAPPA);
comparing the mRNA expression for each of the genes from at least two cumulus cell masses, each of the at least two cumulus cell masses harvested from different oocytes;
selecting the corresponding oocyte associated with one of the at least two cumulus cell masses based on the comparing of the mRNA expression for each of the genes, wherein the corresponding oocyte comprises a euploid oocyte;
wherein a relative decrease of mRNA expression level of AREG and/or HSD3B1 in the cumulus cell mass from the corresponding euploid oocyte compared to another cumulus cell mass from the corresponding euploid oocyte is indicative of a live birth outcome, or
wherein a relative increase of mRNA expression level of PAPPA and/or LHCGR in the cumulus cell mass from the corresponding euploid oocyte compared to another cumulus cell mass from the corresponding euploid oocyte is indicative of a live birth outcome;
fertilizing the selected oocyte in vitro to form an embryo; and
implanting the embryo in a patient.
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