US 11,988,666 B2
Lectin-binding substance measurement method, lectin-binding substance measurement kit, and blocked labeled lectin for use in these
Tomonori Nishii, Tokyo (JP); Kazunori Okada, Hachioji (JP); Naoki Ishikawa, Hachioji (JP); Kumiko Iida, Hachioji (JP); Shintaro Yagi, Hachioji (JP); and Katsumi Aoyagi, Tokyo (JP)
Assigned to FUJIREBIO INC., Tokyo (JP)
Appl. No. 17/639,391
Filed by FUJIREBIO INC., Tokyo (JP)
PCT Filed Sep. 1, 2020, PCT No. PCT/JP2020/033137
§ 371(c)(1), (2) Date Mar. 1, 2022,
PCT Pub. No. WO2021/045061, PCT Pub. Date Mar. 11, 2021.
Claims priority of application No. 2019-159746 (JP), filed on Sep. 2, 2019.
Prior Publication US 2022/0326245 A1, Oct. 13, 2022
Int. Cl. G01N 33/574 (2006.01)
CPC G01N 33/57484 (2013.01) [G01N 2333/471 (2013.01); G01N 2333/4724 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A lectin-binding substance measurement method for measuring a lectin-binding substance in a sample, the method comprising
a measuring step of bringing a blocked labeled lectin and a labeled lectin into contact with the sample, wherein
the blocked labeled lectin is a combination of
a high molecular weight blocked labeled lectin including a first water-soluble carrier made of a high molecular weight water-soluble polymer having a weight average molecular weight of 200,000 or more, and a first labeling substance and a first lectin immobilized on the first water-soluble carrier and
a low molecular weight blocked labeled lectin including a second water-soluble carrier made of a low molecular weight water-soluble polymer having a weight average molecular weight of less than 100,000, and a second labeling substance and a second lectin immobilized on the second water-soluble carrier,
the labeled lectin comprises a third labeling substance and a third lectin, and excludes any water-soluble carrier,
the high molecular weight water-soluble polymer and the low molecular weight water soluble polymer are comprised of a same or different class of polymer,
the first labeling substance, the second labeling substance, and the third labeling substance are a same or different labeling substance, and
the first lectin, the second lectin, and the third lectin are a same or different lectin.