US 11,988,661 B2
Rapid quantitative test to diagnose polycystic kidney disease
Jeffrey Matthijn Beekman, Utrecht (NL); Johanna Florentia Dekkers, Utrecht (NL); Cornelis Korstiaan Van Der Ent, Utrecht (NL); and Johannes Carolus Clevers, Utrecht (NL)
Assigned to UMC Utrecht Holding, B.V., Utrecht (NL); and Koninklijke Nederlandse Akademie van Wetenschappen, Utrecht (NL)
Filed by UMC Utrecht Holding, B.V., Utrecht (NL); and Koninklijke Nederlandse Akademie van Wetenschappen, Utrecht (NL)
Filed on May 7, 2021, as Appl. No. 17/314,557.
Application 17/314,557 is a division of application No. 16/003,293, filed on Jun. 8, 2018, granted, now 11,035,852.
Application 16/003,293 is a continuation of application No. 14/367,061, granted, now 10,006,904, previously published as PCT/IB2012/057497, filed on Dec. 19, 2012.
Claims priority of provisional application 61/579,661, filed on Dec. 23, 2011.
Claims priority of provisional application 61/577,110, filed on Dec. 19, 2011.
Prior Publication US 2021/0333266 A1, Oct. 28, 2021
Int. Cl. G01N 33/50 (2006.01)
CPC G01N 33/5082 (2013.01) [G01N 33/5026 (2013.01); G01N 2500/10 (2013.01); G01N 2800/26 (2013.01); G01N 2800/347 (2013.01); G01N 2800/382 (2013.01); G01N 2800/52 (2013.01)] 5 Claims
 
1. An in vitro method for diagnosing polycystic kidney disease, wherein the method comprises
measuring swelling of one or more organoids generated from primary kidney cells derived from a subject,
wherein swelling means an increase in size of the one or more organoids due to fluid uptake,
and
wherein a larger size of said one or more organoids compared to a control organoid indicates the presence of polycystic kidney disease.