US 11,988,651 B2
Metabolome sampling and analysis method for analyzing metabolome during synthetic gas fermentation of synthetic gas fermentation microorganisms
Kyoung Heon Kim, Seoul (KR); Young Soon Um, Seoul (KR); Jung Yeon Kim, Seoul (KR); and Joongsuk Kim, Gwangmyeong-si (KR)
Assigned to Korea University Research and Business Foundation, Seoul (KR); and Korea Institute of Science and Technology, Seoul (KR)
Appl. No. 16/975,581
Filed by Korea University Research and Business Foundation, Seoul (KR); and Korea Institute of Science and Technology, Seoul (KR)
PCT Filed Feb. 21, 2019, PCT No. PCT/KR2019/002146
§ 371(c)(1), (2) Date Aug. 25, 2020,
PCT Pub. No. WO2019/168300, PCT Pub. Date Sep. 6, 2019.
Claims priority of application No. 10-2018-0024259 (KR), filed on Feb. 28, 2018.
Prior Publication US 2020/0393427 A1, Dec. 17, 2020
Int. Cl. G01N 30/72 (2006.01); G01N 1/22 (2006.01); G01N 30/06 (2006.01); G01N 30/86 (2006.01); G01N 30/88 (2006.01); G01N 33/497 (2006.01)
CPC G01N 30/7206 (2013.01) [G01N 1/22 (2013.01); G01N 30/06 (2013.01); G01N 30/8675 (2013.01); G01N 30/88 (2013.01); G01N 33/497 (2013.01); G01N 2001/2244 (2013.01); G01N 2030/062 (2013.01); G01N 2030/8813 (2013.01); G01N 2033/4977 (2013.01)] 1 Claim
OG exemplary drawing
 
1. A method for analyzing metabolomic differentiation for distinguishing a glucose culture and a synthetic gas culture of Clostridium carboxidivorans, the method comprising:
a metabolome sampling step of subjecting a biological sample of Clostridium carboxidivorans to fast filtration under an aerobic condition, washing the filtrate with water, and then extracting a metabolome using a mixed solvent of water, 2-propanol, and methanol at a volume ratio of 2:2:5 as an extraction solvent.