	US 11,988,633 B2
	Use of polyamines as resolving agents for capillary electrophoresis of labeled glycans using gels
Fielito U. Limjap, Newark, CA (US); Michael J. Kimzey, Oakland, CA (US); and Francis T. Haxo, San Francisco, CA (US)
Assigned to Agilent Technologies, Inc., Santa Clara, CA (US)
Appl. No. 16/644,467
Filed by Agilent Technologies, Inc., Santa Clara, CA (US)
PCT Filed Sep. 20, 2018, PCT No. PCT/US2018/052079 § 371(c)(1), (2) Date Mar. 4, 2020, PCT Pub. No. WO2019/060633, PCT Pub. Date Mar. 28, 2019.
Claims priority of provisional application 62/562,189, filed on Sep. 22, 2017.
Prior Publication US 2021/0063347 A1, Mar. 4, 2021
Int. Cl. G01N 27/447 (2006.01); C08B 37/00 (2006.01); C08G 73/02 (2006.01); C08L 79/02 (2006.01)

CPC G01N 27/44747 (2013.01) [C08B 37/0003 (2013.01); C08B 37/0087 (2013.01); C08G 73/0206 (2013.01); C08G 73/0273 (2013.01); C08L 79/02 (2013.01); G01N 27/44791 (2013.01)]

20 Claims

13. A cartridge or capillary loaded with a gel for capillary electrophoretic separation of labeled glycans, which gel comprises at least one polyamine of any of the following structures:

Structure 1

wherein each of R₁-R₃are independently selected from H and CH₃,

Structure 2

wherein R₁and R₂are independently selected from H and CH₃, and said polyamine is N,N,N′,N′-Tetramethyl-1,3-propanediamine,

Structure 3

wherein each R₁and R₂are independently selected from H and CH₃,

Structure 4

wherein each of R₁-R₃is independently selected from H and CH₃,

Structure 5

wherein each of R₁-R₄is independently selected from H and CH₃,

Structure 6,

wherein each of R₁-R₆is independently selected from H and CH₃,

Structure 7

wherein each of R₁-R₆is independently H or CH₃, or

Structure 8

wherein each of R₁-R₇is independently H or CH₃, provided that, when the cartridge or capillary is a capillary and the gel is of polyethylene oxide (“PEO”), the capillary is coated.